| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL3.3[secNluc/minP] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3235 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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pNL3.3[secNluc/minP]载体质粒基本信息
pNL3.3[secNluc/minP]质粒图谱载体图谱和pNL3.3[secNluc/minP]载体序列质粒序列多克隆位点信息
![pNL3.3[secNluc-minP]载体图谱](/uploads/载体图谱/pNL3.3[secNluc-minP]载体图谱.png)
pNL3.3[secNluc/minP]质粒载体简介
pNL3.3[secNluc/minP]质粒序列载体序列
LOCUS pNL3.3[secNluc minP] 3235 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Vector with a minimal promoter for measuring the activity of
transcriptional response elements using secreted NanoLuc(R)
luciferase.
ACCESSION JQ513371
VERSION .
KEYWORDS pNL3.3[secNluc minP]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3235)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Thursday, February 20, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3235
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
promoter 77..108
/note="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 141..227
/codon_start=1
/product="secretion signal from interleukin 6"
/note="IL-6 signal sequence"
/translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
CDS 228..740
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
LCERILA"
polyA_signal 789..910
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1329..1917)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2117..2977)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3082..3130
/note="synthetic polyadenylation signal"
misc_feature 3144..3235
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
121 gtactgttgg taaagccacc atgaactcct tctccacaag cgccttcggt ccagttgcct
181 tctccctggg cctgctcctg gtgttgcctg ctgccttccc tgccccagtc ttcacactcg
241 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
301 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
361 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
421 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
481 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
541 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
601 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
661 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
721 gcgaacgcat tctggcgtaa ggccgcgact ctagagtcgg ggcggccggc cgcttcgagc
781 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
841 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
901 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
961 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
1021 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
1081 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
1141 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
1201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
1261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
1321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
1381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
1441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
1501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
1561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
1621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
1681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
1741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
1801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
1861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
1921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
1981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
2041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc
2101 taaaccactg cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc
2161 tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg
2221 gcttaccatc aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg
2281 atttgtcagc aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt
2341 tgtccgcctc catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag
2401 tgagtagttt ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt
2461 tcggtatggc ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca
2521 tattatgaag aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg
2581 ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat
2641 ccgtaagatg cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta
2701 tacggcgacc aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca
2761 gtactttgaa agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct
2821 tgccgctatt gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat
2881 cttttacttt caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga
2941 agggaatgag tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt
3001 gaagcattta tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac
3061 gggaggtatt ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt
3121 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
3181 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
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pNL3.3[secNluc/minP]质粒载体应用举例
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